The study concluded that P. damselae: “KPD is the causative agent of myonecrosis and hepatopancreatic necrosis, representing a threat in hatcheries in India”.
A summary of a study published in Aquaculture International said, this is the first report of Bacterial myonecrosis and Hepatopancreatic necrosis in broodstock (Litopenaeus vannamei) due to caused by bacteria. Of the 400 individuals examined at shrimp hatcheries in India, 34 exhibited myonecrosis and hepatopancreatic necrosis, previously reported conditions due to bacterial, fungal, parasitic and viral infections. Outward signs of infection include passive swimming, anorexia, and cloudy abdominal muscles with extensive white discoloration of the distal abdomen and posterior appendages. Visually surgical visual examination revealed atrophic hepatopancreas, empty stomach and middle intestine. Histopathological studies show reddening of muscle lesions consisting of coagulant necrosis and water necrosis of striated muscle fibers, often edema. The hepatopancreas has necrotic symptoms such as a structural disruption of the cavity and the diffuse tubular epithelial cells over the necrotic muscle epithelial cells. The gills in infected shrimp are disordered, tiny filaments are absorbed by the inflammation due to red blood cells. RT-PCR analysis using primers as recommended by the OIE and additional primers that have been used in previous studies showed that there is no IMN virus or other emerging pathogens such as MrNV, PvNV, etc. WSSV, IHHNV, TSV, YHV, AHPND and EHP. Based on the above negative results, microbiological tests were conducted. The predominant yellow capture colonies were isolated on TCBS agar and identified as Photobacterium damselae subsp. damselae KPD. Infection by injection method P. damselae KPD has proven that this is a pathogen that has infected with L. vannamei parents.
How to prevent and treat diseases
Cleaning pond clean, dredging sludge and drying pond bottom before putting in nursery.
The stocking density should not be too thick to ensure enough oxygen.
Use aerators to promptly supplement the pond.
Combined with APC-YUCCA with a dose of 500g / 1000m3 of water.
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